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Image Search Results
Journal: Biochemistry and Biophysics Reports
Article Title: HUC-MSC-derived exosomal miR-16-5p attenuates inflammation via dual suppression of M1 macrophage polarization and Th1 differentiation
doi: 10.1016/j.bbrep.2025.102078
Figure Lengend Snippet: miR-16-5p mimics blocks M1 macrophage polarization and Th1 cell differentiation. A: Raw264.7 cells were transfected with miR-16-5p mimics or NC mimics and subsequently induced to M1 macrophage polarization. After 24 h of culture, the percentage of CD11b + iNOS + cells in each group were measured by flow cytometry. B: Data are presented as mean ± standard deviation ( x ‾ ± s), with n = 3. p < 0.0001 compared with M1 group and NC mimics group. C: Naïve CD4 + T cells were induced to Th1 cells and then transfected with miR-16-5p or NC mimic. After 5 days of culture, the percentage of CD4 + IFN-γ + cells in each group were measured by flow cytometry. D: Data are presented as mean ± standard deviation ( x ‾ ±s), with n = 3. p = 0.0319, compared with Th1 group p = 0.0237, compared with NC mimics group. E: The inhibitory effect of miR-16-5p transfection on the mRNA levels of iNOS and TNF-α in M1 cells was measured by q-PCR. Data are presented as mean ± standard deviation ( x ‾ ±s), with n = 3. p iNOS <0.0001, p TNF-α = 0.0038, compared with M1 group, p iNOS <0.0001, p TNF-α = 0.0002, compared with NC mimics group. F: The mRNA expression levels of IFN-γ and STAT4 in different groups were measured by q-PCR. Data are presented as mean ± standard deviation ( x ‾ ±s), with n = 3. p STAT1 = 0.0018, p I - γ = 0.0032, compared with Th1 group, p STAT1 <0.0001, p I - γ = 0.0286, compared with NC mimics group.
Article Snippet: The single cells were labeled with iNOS (APC-iNOS, #17592082, eBioscience), F4/80 (FITC-F4/80, #35-4801-U100, Tonbo Biosciences), CD11b (PerCP-Cyanine5.5-CD11b, 65-0112-U100, Tonbo Biosciences), CD45(PE-CD45, #147712, Biolegend), CD3 (FITC-CD3, #35-0032-U100, Tonbo Biosciences) and
Techniques: Cell Differentiation, Transfection, Flow Cytometry, Standard Deviation, Expressing
Journal: Biochemistry and Biophysics Reports
Article Title: HUC-MSC-derived exosomal miR-16-5p attenuates inflammation via dual suppression of M1 macrophage polarization and Th1 differentiation
doi: 10.1016/j.bbrep.2025.102078
Figure Lengend Snippet: miR-34a-5p and miR-125b-5p mimics suppress M1 macrophage polarization and Th1 cell differentiation. A: The Raw264.7 cells were transfected miR-34a-5p mimic, miR-125b-5p mimics or NC mimics and subsequently induced to M1 macrophage. After 24 h of culture, the percentage of CD11b + iNOS + cells in various group was measured by flow cytometry. B: Data are presented as mean ± standard deviation ( x ‾ ± s), with n = 3. p M1,34 = 0.0118, p M1,125 = 0.0037, compared with M1 group; p NC,34 = 0.0202, p NC,125 = 0.0060, compared with NC mimics group. C: Naïve CD4 + T cells were induced to Th1 cells and then transfected with miR-34a-5p mimics, miR-125b-5p mimics or NC mimic. After 5 days of culture, the percentage of CD4 + IFN-γ + cells in various groups was measured by flow cytometry. D: Data are presented as mean ± standard deviation ( x ‾ ± s), with n = 3. p Th1,34 = 0.0102, p Th1,125 = 0.0201, compared with Th1 group, p NC,34 = 0.0220, p NC,125 = 0.0447, compared with NC mimics group. E: The inhibitory effects of miR-34a-5p mimics or miR-125b-5p mimics transfection on the mRNA levels of iNOS and TNF-α in M1 cells was measured by q-PCR. Data are presented as mean ± standard deviation ( x ‾ ± s), with n = 3. p 34-iNOS <0.0001, p 125-iNOS <0.0001, p 34-TNF-α = 0.0012, p 125-TNF-α = 0.0117, compared with M1 group, p 34-iNOS = 0.0001, p 125-iNOS = 0.0027, p 34-TNF-α = 0.0039, p 125-TNF-α = 0.0377, compared with NC mimics group. F: The mRNA expression level of I - γ and STAT1 in different groups were measured by q-PCR. x ‾ ± s, with n = 3. p 34-STAT1 <0.0001, p 125-STAT1 <0.0001, p 3 - γ <0.0001, p 1 - γ <0.0001, compared with Th1 group, p 34-STAT1 <0.0001, p 125-STAT1 <0.0001, p 3 - γ = 0.0014, p 1 - γ = 0.0004, compared with NC mimics group.
Article Snippet: The single cells were labeled with iNOS (APC-iNOS, #17592082, eBioscience), F4/80 (FITC-F4/80, #35-4801-U100, Tonbo Biosciences), CD11b (PerCP-Cyanine5.5-CD11b, 65-0112-U100, Tonbo Biosciences), CD45(PE-CD45, #147712, Biolegend), CD3 (FITC-CD3, #35-0032-U100, Tonbo Biosciences) and
Techniques: Cell Differentiation, Transfection, Flow Cytometry, Standard Deviation, Expressing